Nanoindentation of Mesenchymal Stem Cells using Atomic Force Microscopy: Effect of Adhesive Cell-Substrate Structures
Résumé
The procedure commonly adopted to characterize cell materials using atomic force
microscopy neglects the stress state induced in the cell by the adhesion structures that
anchor it to the substrate. In several studies, the cell is considered as made from a single
material and no specific information is provided regarding the mechanical properties of
subcellular components. Here we present an optimization algorithm to determine separately
the material properties of subcellular components of mesenchymal stem cells subjected to
nanoindentation measurements. We assess how these properties change if the adhesion
structures at the cell-substrate interface are considered or not in the algorithm. In particular,
among the adhesion structures, the focal adhesions and the stress fibers were simulated. We
found that neglecting the adhesion structures leads to underestimate the cell mechanical
properties thus making errors up to 15%. This result leads us to conclude that the action of
adhesion structures should be taken into account in nanoindentation measurements
especially for cells that include a large number of adhesions to the substrate.
Fichier principal
Migliorini+et+al_2021_Nanotechnology_10.1088_1361-6528_abe748.pdf (3.98 Mo)
Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)