Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces

Valerie de Crecy-Lagard (1) , Pascale Servant-Moisson (1) , Julie Viala (1) , Cosette Grandvalet (1) , Philippe Mazodier (1)

1 Biochimie Microbienne

The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP-dependent protease family Clp were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2, located in tandem upstream from the clpX gene, and at least two unlinked clpC genes. Disruption of the clpP1 gene in S. lividans and S. coelicolor blocks differentiation at the substrate mycelium step. Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans, S. albus and S. coelicolor. Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans.

Domaines: Biochimie, Biologie Moléculaire

Valerie de Crecy-Lagard, Pascale Servant-Moisson, Julie Viala, Cosette Grandvalet, Philippe Mazodier. Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces. Molecular Microbiology, 1999, 32 (3), pp.505-517. ⟨10.1046/j.1365-2958.1999.01364.x⟩. ⟨hal-03140074⟩