Identification and determination of selenohomolanthionine – The major selenium compound in Torula yeast

Katarzyna Bierla (1) , Noriyuki Suzuki (2) , Yasumitsu Ogra (2) , Joanna Szpunar (1) , Ryszard Lobinski (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
2 Chiba University

Torula yeast (Candida utilis) was found to metabolize selenium in a totally different way to Brewer's yeast (S. cerevisiae) leading to the biosynthesis of selenohomolanthionine (SeHLan), a major selenium compound accounting for 60-80% of the total selenium. The identity of SeHLan was confirmed by retention time matching in hydrophilic ion interaction chromatography (HILIC) with inductively coupled plasma mass spectrometric detection (ICP MS) using a custom synthesized standard molecule and by HILIC-Orbitrap MS and MS-MS fragmentation. Selenohomolanthionine escapes the current assays for the organic character of Se-rich yeast based on the protein-bound selenomethionine determination. A HILIC-ICP MS method was developed for the quantitative determination of selenohomolanthionine in yeast supplements with a detection limit of 146 ng/g.

Mots clés: HPLC-ICP MS; Selenohomolanthionine; Yeast; Food supplement; HPLC-ESI; Orbitrap MS

Domaines: Chimie analytique; Ingénierie des aliments

Katarzyna Bierla, Noriyuki Suzuki, Yasumitsu Ogra, Joanna Szpunar, Ryszard Lobinski. Identification and determination of selenohomolanthionine – The major selenium compound in Torula yeast. Food Chemistry, 2017, 237, pp.1196-1201. ⟨10.1016/j.foodchem.2017.06.042⟩. ⟨hal-03133557⟩