Antimicrobial Peptide K11 Selectively Recognizes Bacterial Biomimetic Membranes and Acts by Twisting Their Bilayers

Francisco Ramos-Martín (1) , Claudia Herrera-León (1) , Viviane Antonietti (2) , Pascal Sonnet (2) , Catherine Sarazin (1) , Nicola D’amelio (1)

1 GEC - Génie Enzymatique et Cellulaire
2 AGIR - Agents infectieux, résistance et chimiothérapie - UR UPJV 4294

K11 is a synthetic peptide originating from the introduction of a lysine residue in position 11 within the sequence of a rationally designed antibacterial scaffold. Despite its remarkable antibacterial properties towards many ESKAPE bacteria and its optimal therapeutic index (320), a detailed description of its mechanism of action is missing. As most antimicrobial peptides act by destabilizing the membranes of the target organisms, we investigated the interaction of K11 with biomimetic membranes of various phospholipid compositions by liquid and solid-state NMR. Our data show that K11 can selectively destabilize bacterial biomimetic membranes and torque the surface of their bilayers. The same is observed for membranes containing other negatively charged phospholipids which might suggest additional biological activities. Molecular dynamic simulations reveal that K11 can penetrate the membrane in four steps: after binding to phosphate groups by means of the lysine residue at the N-terminus (anchoring), three couples of lysine residues act subsequently to exert a torque in the membrane (twisting) which allows the insertion of aromatic side chains at both termini (insertion) eventually leading to the flip of the amphipathic helix inside the bilayer core (helix flip and internalization)

Domaines: Pharmacologie; Chimie théorique et/ou physique

Francisco Ramos-Martín, Claudia Herrera-León, Viviane Antonietti, Pascal Sonnet, Catherine Sarazin, et al.. Antimicrobial Peptide K11 Selectively Recognizes Bacterial Biomimetic Membranes and Acts by Twisting Their Bilayers. Pharmaceuticals, 2021, 14 (1), pp.1. ⟨10.3390/ph14010001⟩. ⟨hal-03126936⟩