Influence of chromatin condensation on the numberof direct dsb damages induced by ions studied using a monte carlo code

M. Dos Santos (1) , I. Clairand (1) , G. Gruel (1) , J.F. Barquinero (1) , S. Incerti (2) , C. Villagrasa (1)

1 IRSN - Institut de Radioprotection et de Sûreté Nucléaire
2 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan

The purpose of this work is to evaluate the influence of the chromatin condensation on the number of direct double-strand break (DSB) damages induced by ions. Two geometries of chromosome territories containing either condensed or decondensed chromatin were implemented as biological targets in the Geant4 Monte Carlo simulation code and proton and alpha irradiation was simulated using the Geant4-DNA processes. A DBSCAN algorithm was used in order to detect energy deposition clusters that could give rise to single-strand breaks or DSBs on the DNA molecule. The results of this study show an increase in the number and complexity of DNA DSBs in condensed chromatin when compared with decondensed chromatin. © The Author 2014.

Mots clés: chromatin; DNA; ion; nucleosome; proton; algorithm; alpha radiation; animal; chemistry; chromatin; chromosome; cluster analysis; computer program; computer simulation; DNA damage; double stranded DNA break; electron; human; linear energy transfer; Monte Carlo method; ultrastructure; Algorithms; Alpha Particles; Animals; Chromatin; Chromosomes; Cluster Analysis; Computer Simulation; DNA Breaks; Double-Stranded; DNA Damage; Electrons; Humans; Ions; Linear Energy Transfer; Monte Carlo Method; Nucleosomes; Protons; Software

Domaines: Sciences du Vivant [q-bio]

M. Dos Santos, I. Clairand, G. Gruel, J.F. Barquinero, S. Incerti, et al.. Influence of chromatin condensation on the numberof direct dsb damages induced by ions studied using a monte carlo code. RADIATION PROTECTION DOSIMETRY, 2014, 161 (1-4), pp.469-473. ⟨10.1093/rpd/ncu029⟩. ⟨hal-03118952⟩