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In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging

Abstract : The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustaceanGammarus fossarumusing matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high-resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.
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https://hal.archives-ouvertes.fr/hal-03113280
Contributor : Olivier Geffard <>
Submitted on : Monday, January 18, 2021 - 11:39:24 AM
Last modification on : Monday, July 26, 2021 - 3:03:01 PM

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Tingting Fu, Janina Oetjen, Manuel Chapelle, Alexandre Verdu, Matthias Szesny, et al.. In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging. Journal of Mass Spectrometry, Wiley-Blackwell, 2020, 55 (9), pp.e4531. ⟨10.1002/jms.4531⟩. ⟨hal-03113280⟩

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