A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis

Nathalie Ollivier (1) , Jérôme Vicogne (1) , Aurélie Vallin (1) , Hervé Drobecq (1) , Rémi Desmet (1) , Ouafâa El mahdi (2) , Bérénice Leclercq (1) , Gautier Goormachtigh (1) , Véronique Fafeur (1) , Oleg Melnyk (1)

1 IBLI - Institut de biologie de Lille - IBL
2 USMBA - Université Sidi Mohamed Ben Abdellah

Native chemical ligation (NCL) and bis(2-sulfanylethyl)amido (SEA) ligation allow the one-pot assembly of three peptide segments in the N-to-C direction. The SEA group (see picture, blue) is switched off by intramolecular disulfide bond formation during NCL. Then, a phosphine switches it on to trigger the second SEA ligation step. The K1 domain of the hepatocyte growth factor was synthesized and found to be biologically active.

Mots clés: growth factors; native chemical ligation; proteins; solid-phase synthesis; synthetic methods

Domaines: Biotechnologies; Chimie organique; Polymères; Biochimie [q-bio.BM]

Nathalie Ollivier, Jérôme Vicogne, Aurélie Vallin, Hervé Drobecq, Rémi Desmet, et al.. A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis. Angewandte Chemie International Edition, 2011, 51 (1), pp.209-213. ⟨10.1002/anie.201105837⟩. ⟨hal-03104069⟩