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Artificial maturation of [FeFe] hydrogenase in a redox polymer film

Abstract : We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach clears further the way to the implementation of hydrogenases in large scale real life processes.
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Submitted on : Thursday, January 7, 2021 - 5:20:53 PM
Last modification on : Wednesday, November 9, 2022 - 4:41:10 PM
Long-term archiving on: : Thursday, April 8, 2021 - 7:45:02 PM


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Christina Felbek, Steffen Hardt, Cecilia Papini, Debajyoti Pramanik, Vincent Artero, et al.. Artificial maturation of [FeFe] hydrogenase in a redox polymer film. Chemical Communications, 2021, 57 (14), pp.1750-1753. ⟨10.1039/d0cc08168j⟩. ⟨hal-03102775⟩



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