In Vitro/Ex Vivo Models for the Study of Ischemia Reperfusion Injury during Kidney Perfusion

Sebastien Giraud (1, 2) , Raphael Thuillier (1, 2, 3) , Jérome Cau (1, 3, 2) , Thierry Hauet (1, 3, 2, 4, 5)

1 IRTOMIT [Poitiers] - Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques [U 1082]
2 CHU de Poitiers [La Milétrie] - Centre hospitalier universitaire de Poitiers = Poitiers University Hospital
3 Université de Poitiers - Faculté de Médecine et de Pharmacie
4 IBiSA Plateforme, Plate-forme MOdélisation Préclinique - Innovation Chirurgicale et Technologique (MOPICT)
5 FHU SUPORT - Fédération Hospitalo-universitaire SUrvival oPtimization in ORgan Transplantation

Oxidative stress is a key element of ischemia–reperfusion injury, occurring during kidney preservation and transplantation. Current options for kidney graft preservation prior to transplantation are static cold storage (CS) and hypothermic machine perfusion (HMP), the latter demonstrating clear improvement of preservation quality, particularly for marginal donors, such as extended criteria donors (ECDs) and donation after circulatory death (DCDs). Nevertheless, complications still exist, fostering the need to improve kidney preservation. This review highlights the most promising avenues of in kidney perfusion improvement on two critical aspects: ex vivo and in vitro evaluation.

Mots clés: kidney; in vitro; oxidative stress; perfusion; oxygenation

Domaines: Médecine humaine et pathologie

Sebastien Giraud, Raphael Thuillier, Jérome Cau, Thierry Hauet. In Vitro/Ex Vivo Models for the Study of Ischemia Reperfusion Injury during Kidney Perfusion. International Journal of Molecular Sciences, 2020, 21 (21), pp.8156. ⟨10.3390/ijms21218156⟩. ⟨hal-03102520⟩