Cell Permeabilization and Molecular Delivery Following Helium Plasma jet Treatments
Résumé
Molecular delivery in cells combining low cytotoxicity and high efficiency using physical techniques remains to be optimized In this work, we report on the use of a cold atmospheric pressure plasma jet device called Plasma Gun as an innovative strategy for cell membrane permeabilization In our previous study[ 1 we focused on evaluating key parameters needed to achieve an efficient molecular delivery We showed that a low number of pulses (between 1 000 and 100 000 and a 30 minute incubation at 37 C of the treated cells were enhancing molecular uptake in a ring shape pattern with no significant toxicity In this report, we investigated cell permeabilization kinetics and surprisingly, best permeabilization levels were reached when molecular injections in the cell medium and plasma treatments were not simultaneous Indeed, best efficiency was measured when the injection was performed a few minutes after plasma This transient permeabilization was also measured for longer delays of molecular injection which confirms the non toxic nature of our plasma treatment conditions
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IMPCS-1 2019 Poster Vinodini Vijayarangan et al.pdf (1.4 Mo)
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