Extracellular IL-33 cytokine, but not endogenous nuclear IL-33, regulates protein expression in endothelial cells

Violette Gautier (1) , Corinne Cayrol (1) , Dorian Farache (1) , Stéphane Roga (1) , Bernard Monsarrat (1) , Odile Burlet-Schiltz (1) , Anne Gonzalez de Peredo (1) , Jean-Philippe Girard (1)

1 IPBS - Institut de pharmacologie et de biologie structurale

IL-33 is a nuclear cytokine from the IL-1 family that plays important roles in health and disease. Extracellular IL-33 activates a growing number of target cells, including group 2 innate lymphoid cells, mast cells and regulatory T cells, but it remains unclear whether intracellular nuclear IL-33 has additional functions in the nucleus. Here, we used a global proteomic approach based on high-resolution mass spectrometry to compare the extracellular and intracellular roles of IL-33 in primary human endothelial cells, a major source of IL-33 protein in human tissues. We found that exogenous extracellular IL-33 cytokine induced expression of a distinct set of proteins associated with inflammatory responses in endothelial cells. In contrast, knockdown of endogenous nuclear IL-33 expression using two independent RNA silencing strategies had no reproducible effect on the endothelial cell proteome. These results suggest that IL-33 acts as a cytokine but not as a nuclear factor regulating gene expression in endothelial cells.

Domaines: Sciences du Vivant [q-bio]

Violette Gautier, Corinne Cayrol, Dorian Farache, Stéphane Roga, Bernard Monsarrat, et al.. Extracellular IL-33 cytokine, but not endogenous nuclear IL-33, regulates protein expression in endothelial cells. Scientific Reports, 2016, 6 (1), ⟨10.1038/srep34255⟩. ⟨hal-03080087⟩