Skip to Main content Skip to Navigation
Journal articles

Osteogenic-differentiated Mesenchymal Stem Cell-secreted Extracellular Matrix as a Bone Morphogenetic Protein-2 delivery system for ectopic bone formation

Abstract : While human bone morphogenetic protein-2 (BMP-2) is a promising growth factor for bone regeneration, a major challenge in biomedical applications is finding an optimal carrier for its delivery at the site of injury. Because of their natural affinities for growth factors (including BMP-2) as well as their role in instructing cell function, cultured cell-derived extracellular matrices (ECM) are of special interest. We hereby hypothesized that a “bony matrix” containing mineralized, osteogenic ECM is a potential efficacious carrier of BMP-2 for promoting bone formation and, therefore, compared the efficacy of the decellularized ECM derived from osteogenic-differentiated human mesenchymal stem cells (hMSCs) to the one obtained from ECM from undifferentiated hMSCs. Our results provided evidence that both ECMs can bind BMP-2 and promote bone formation when implanted ectopically in mice. The osteoinductive potential of BMP-2, however, was greater when loaded within an osteogenic MSC-derived ECM; this outcome was correlated with higher sequestration capacity of BMP-2 over time in vivo. Interestingly, although the BMP-2 mainly bound onto the mineral crystals contained within the osteogenic MSC derived-ECM, these mineral components were not involved in the observed higher osteoinductivity, suggesting that the organic components were the critical components for the matrix efficacy as BMP-2 carrier.
Keywords : dissemin
Complete list of metadata

https://hal-univ-paris.archives-ouvertes.fr/hal-03077271
Contributor : Delphine Logeart-Avramoglou Connect in order to contact the contributor
Submitted on : Tuesday, October 12, 2021 - 10:01:45 AM
Last modification on : Monday, October 18, 2021 - 3:24:21 PM

File

article.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Nathanael Larochette, Hanane El-Hafci, Esther Potier, Niclas Setterblad, Morad Bensidhoum, et al.. Osteogenic-differentiated Mesenchymal Stem Cell-secreted Extracellular Matrix as a Bone Morphogenetic Protein-2 delivery system for ectopic bone formation. ACTA BIOMATERIALIA, 2020, 116, pp.186-200. ⟨10.1016/j.actbio.2020.09.003⟩. ⟨hal-03077271⟩

Share

Metrics

Record views

32

Files downloads

4