Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Béatrice Vallée (1) , Michel Doudeau (1) , Fabienne Godin (1) , Hélène Bénédetti (1)

1 CBM - Centre de biophysique moléculaire

We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.

Domaines: Biochimie [q-bio.BM]

Béatrice Vallée, Michel Doudeau, Fabienne Godin, Hélène Bénédetti. Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein. Journal of visualized experiments : JoVE, 2019, 148, pp.e59820. ⟨10.3791/59820⟩. ⟨hal-02282630⟩