Nanoparticle detection by solid state nanopore integrated into a reusable microfluidic device

Izadora Mayumi Fujinami Tanimoto (1) , Benjamin Cressiot (1) , Jean Roman (2) , Nathalie Jarroux (1) , Gilles Patriarche (3) , Bruno Le Pioufle (2) , Juan Pelta (1) , Laurent Bacri (1)

1 LAMBE - UMR 8587 - Laboratoire Analyse, Modélisation et Matériaux pour la Biologie et l'Environnement
2 IDA - Institut d'Alembert
3 C2N - Centre de Nanosciences et de Nanotechnologies

Solid-state nanopores can be used as electrical biosensors at the single molecule resolution. Such high resolution sensors provide a promising approach to address societal challenges in health and biotechnology. Nevertheless, the analytes detection is still limited by non-specific interactions between these molecules and the membrane supporting the nanopore. Therefore, we performed a polymer functionalization of the nanopore surface that allows to passivate the membrane and to control the pore size. Moreover, we integrated the solid-state nanopore in a reusable microfluidic device, thereby reducing the sample volume needed to perform the detection. The combination of these two approaches is applied to the sensing of single thyroglobulin protein.

Mots clés: Solid-state nanopore; microfluidics; protein detection; polymer functionalization

Domaines: Micro et nanotechnologies/Microélectronique

Izadora Mayumi Fujinami Tanimoto, Benjamin Cressiot, Jean Roman, Nathalie Jarroux, Gilles Patriarche, et al.. Nanoparticle detection by solid state nanopore integrated into a reusable microfluidic device. MicroTAS, Oct 2020, on-line, France. ⟨hal-03031395⟩