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Article Dans Une Revue Cell Reports Année : 2020

Degradation of Non-coding RNAs Promotes Recycling of Termination Factors at Sites of Transcription

Tommaso Villa
Mara Barucco
  • Fonction : Auteur
Maria-Jose Martin-Niclos
  • Fonction : Auteur
Alain Jacquier
Domenico Libri

Résumé

A large share of the non-coding transcriptome in yeast is controlled by the Nrd1-Nab3-Sen1 (NNS) complex, which promotes transcription termination of non-coding RNA (ncRNA) genes, and by the nuclear exosome, which limits the steady-state levels of the transcripts produced. How unconstrained ncRNA levels affect RNA metabolism and gene expression are long-standing and important questions. Here, we show that degradation of ncRNAs by the exosome is required for freeing Nrd1 and Nab3 from the released transcript after termination. In exosome mutants, these factors are sequestered by ncRNAs and cannot be efficiently recycled to sites of transcription, inducing termination defects at NNS targets. ncRNA-dependent, genome-wide termination defects can be recapitulated by the expression of a degradation-resistant, circular RNA containing a natural NNS target in exosome-proficient cells. Our results have important implications for the mechanism of termination, the general impact of ncRNAs abundance, and the importance of nuclear ncRNA degradation.
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Dates et versions

hal-03025403 , version 1 (26-11-2020)

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Tommaso Villa, Mara Barucco, Maria-Jose Martin-Niclos, Alain Jacquier, Domenico Libri. Degradation of Non-coding RNAs Promotes Recycling of Termination Factors at Sites of Transcription. Cell Reports, 2020, 32 (3), pp.107942. ⟨10.1016/j.celrep.2020.107942⟩. ⟨hal-03025403⟩
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