Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron

Sylvain Aumonier (1, 2) , Gianluca Santoni (1) , Guillaume Gotthard (1) , David von Stetten (1) , Gordon A. Leonard (1) , Antoine Royant (2, 1)

1 ESRF - European Synchrotron Radiation Facility
2 IBS - UMR 5075 - Institut de biologie structurale

The recent development of serial crystallography has popularized time-resolvedcrystallography as a technique to determine the structure of protein-reactionintermediate states. However, most approaches rely on the availability ofthousands to millions of microcrystals. A method is reported here, usingmonochromatic synchrotron radiation, for the room-temperature collection,processing and merging of X-ray oscillation diffraction data from <100 samplesin order to observe the build up of a photoreaction intermediate species. Usingthis method, we monitored with a time resolution of 63 ms how the populationof a blue-light photoreceptor domain in a crystal progressively photoconvertsfrom the dark to the light state. The series of resulting snapshots allows us tovisualize in detail the gradual rearrangement of both the protein andchromophore during this process.

Mots clés: room-temperature oscillation X-raycrystallography; time-resolved crystallography; serial crystallography; plant photoreceptors

Domaines: Biologie structurale [q-bio.BM]

Sylvain Aumonier, Gianluca Santoni, Guillaume Gotthard, David von Stetten, Gordon A. Leonard, et al.. Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron. International Union of Crystallography journal, 2020, 7 (4), pp.728-736. ⟨10.1107/S2052252520007411⟩. ⟨hal-03014981⟩