Skip to Main content Skip to Navigation
Journal articles

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Abstract : Biomineralization induced by microbial enzymes, which catalyse CaCO3 precipitation, is a promising field of research for various applications in building eco-materials. Especially, this could provide an eco-friendly process for protection of coastal areas against erosion. In the present investigation, fourteen bacterial strains were isolated and characterized from both natural seawater and calcareous deposits formed on a cathodically protected steel mesh in marine environment. All of them induced calcium carbonate precipitation in various media by producing urease and/or carbonic anhydrase enzymes. The calcium carbonate minerals produced by bacteria were identified by microscopy and µ-Raman spectroscopy. In parallel, an experimental set-up, based on a column reactor, was developed to study biomineralization and microbial capacity of Sporosarcina pasteurii to form sandy agglomerate. These well-known calcifying bacteria degraded the urea present in liquid medium circulating through the column to produce calcium carbonate, which acted as cement between sand particles. The bio-bricks obtained after 3 weeks had a compressive strength of 4.2 MPa. 20% of the inter-granular voids were filled by calcite and corresponded to 13% of the total mass. We successfully showed that bio-column system can be used to evaluate the bacterial ability to agglomerate a sandy matrix with CaCO3.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03014433
Contributor : Edp Sciences <>
Submitted on : Thursday, November 19, 2020 - 2:02:24 PM
Last modification on : Wednesday, January 13, 2021 - 3:38:26 AM

File

mt190084.pdf
Publication funded by an institution

Identifiers

Citation

Julia Vincent, René Sabot, Isabelle Lanneluc, Philippe Refait, Philippe Turcry, et al.. Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits. Matériaux & Techniques, EDP Sciences, 2020, 108 (3), pp.302. ⟨10.1051/mattech/2020027⟩. ⟨hal-03014433⟩

Share

Metrics

Record views

86

Files downloads

406