Hypoxia triggers collective aerotactic migration in Dictyostelium discoideum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue eLife Année : 2021

Hypoxia triggers collective aerotactic migration in Dictyostelium discoideum

Résumé

It is well known that eukaryotic cells can sense oxygen (O 2 ) and adapt their metabolism accordingly. It is less known that they can also move towards regions of higher oxygen level (aerotaxis). Using a self-generated hypoxic assay, we show that the social amoeba Dictyostelium discoideum displays a spectacular aerotactic behavior. When a cell colony is covered by a coverglass, cells quickly consume the available O 2 and the ones close to the periphery move directionally outward forming a dense ring keeping a constant speed and density. To confirm that O 2 is the main molecular player in this seemingly collective process, we combined two technological developments, porphyrin based O 2 sensing films and microfluidic O 2 gradient generators. We showed that Dictyostelium cells exhibit aerotactic and aerokinetic (increased speed at low O 2 ) response in an extremely low range of O 2 concentration (0-1.5%) indicative of a very efficient detection mechanism. The various cell behaviors under self-generated or imposed O 2 gradients were modeled with a very satisfactory quantitative agreement using an in silico cellular Potts model built on experimental observations. This computational model was complemented with a parsimonious ‘Go or Grow’ partial differential equation (PDE) model. In both models, we found that the collective migration of a dense ring can be explained by the interplay between cell division and the modulation of aerotaxis, without the need for cell-cell communication. Explicit wave solutions of the PDE model also informed about the relative contributions of division and directed motion on the collective speed.

Domaines

Protistologie
Fichier principal
Vignette du fichier
elife-64731-v1.pdf (5.43 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02997747 , version 1 (12-11-2020)
hal-02997747 , version 2 (02-09-2021)

Identifiants

Citer

Olivier Cochet-Escartin, Mete Demircigil, Satomi Hirose, Blandine Allais, Philippe Gonzalo, et al.. Hypoxia triggers collective aerotactic migration in Dictyostelium discoideum. eLife, 2021, 10, pp.e64731. ⟨10.7554/elife.64731⟩. ⟨hal-02997747v2⟩
111 Consultations
58 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More