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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2020

Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses

Résumé

The nanoscale co-organization of neurotransmitter receptors facing presynaptic release sites is a fundamental determinant of their coactivation and of synaptic physiology. At excitatory synapses, how endogenous AMPARs, NMDARs, and mGluRs are co-organized inside the synapse and their respective activation during glutamate release are still unclear. Combining single-molecule superresolution microscopy, electrophysiology, and modeling, we determined the average quantity of each glutamate receptor type, their nanoscale organization, and their respective activation. We observed that NMDARs form a unique cluster mainly at the center of the PSD, while AMPARs segregate in clusters surrounding the NMDARs. mGluR5 presents a different organization and is homogenously dispersed at the synaptic surface. From these results, we build a model predicting the synaptic transmission properties of a unitary synapse, allowing better understanding of synaptic physiology.

Dates et versions

hal-02986474 , version 1 (03-11-2020)

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Julia Goncalves, Tomas Bartol, Côme Camus, Florian Levet, Ana Paula Menegolla, et al.. Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (25), pp.14503-14511. ⟨10.1073/pnas.1922563117⟩. ⟨hal-02986474⟩
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