Using 13C to Quantify Phloem Transport on Tall Plants in the Field

Daniel Epron (1, 2) , Masako Dannoura (1) , Caroline Plain (2)

1 Kyoto University
2 SILVA - SILVA

The difference in time lags between a labeling pulse of (13) CO2 of the foliage and the appearance of labeled C in the respiration at different locations along the stem of a tall plant is used to estimate at which velocities the isotope tracer, i.e., the labeled carbohydrates, are transported in the phloem sap. Here we describe a method for pulse-labeling tall plants in the field and subsequently tracing C-13 in the respiratory efflux of CO2.

Mots clés: Carbon transfer; Carbon allocation; Laser absorption spectroscopy; Phloem; Pulse labeling; Stable carbon isotope; Stem CO2 efflux

Domaines: Sciences du Vivant [q-bio]; Sciences de l'environnement

Daniel Epron, Masako Dannoura, Caroline Plain. Using 13C to Quantify Phloem Transport on Tall Plants in the Field. Phloem Methods and Protocols, pp.145-151, 2019, ⟨10.1007/978-1-4939-9562-2_12⟩. ⟨hal-02944178⟩