Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis

Jean-Sebastien Guez (1) , François Coutte (2) , Anne-Sophie Drucbert (3) , Pierre-Marie Danzé (3) , Philippe Jacques (2)

1 IP - Institut Pascal
2 Université de Lille
3 IMPRT - Institut de médecine predictive et de recherche thérapeutique

Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, diYculties to isolate RNA attributed to the cell wall were overcome, Wnally authorising powerful RT-PCR's.

Mots clés: RNA isolation; Bacillus subtilis; Natural strain; Cell wall; Transcript analysis; RT-PCR; natural strain; cell wall; transcript analysis

Domaines: Bactériologie; Biotechnologies; Biochimie [q-bio.BM]; Biologie moléculaire; Génie des procédés

Jean-Sebastien Guez, François Coutte, Anne-Sophie Drucbert, Pierre-Marie Danzé, Philippe Jacques. Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis. Archives of Microbiology, 2009, 191, pp.669 - 673. ⟨10.1007/s00203-009-0487-6⟩. ⟨hal-02938860⟩