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Article Dans Une Revue Journal of Biological Chemistry Année : 2020

Turnip yellow mosaic virus protease binds ubiquitin suboptimally to fine-tune its deubiquitinase activity

Résumé

Single-stranded, positive-sense RNA viruses assemble their replication complexes in infected cells from a multi-domain replication polyprotein. This polyprotein usually contains at least one protease whose primary function is to process the polyprotein into mature proteins. Such proteases also may have other functions in the replication cycle. For instance, cysteine proteases (PRO) frequently double up as ubiquitin hydrolases (DUB), thus interfering with cellular processes critical for virus replication. We previously reported the crystal structures of such a PRO/DUB from Turnip yellow mosaic virus (TYMV) and of its complex with one of its PRO substrates. Here we report the crystal structure of TYMV PRO/DUB in complex with ubiquitin. We find that PRO/DUB recognizes ubiquitin in an unorthodox way: It interacts with the body of ubiquitin through a split recognition motif engaging both the major and the secondary recognition patches of ubiquitin (Ile44 patch and Ile36 patch, respectively, including Leu8 which is part of the two patches). However, the contacts are suboptimal on both sides. Introducing a single point mutation in TYMV PRO/DUB aimed at improving Ub-binding led to a much more active DUB. Comparison with other PRO/DUBs from other viral families, particularly coronaviruses, suggests that low DUB activities of viral PRO/DUBs may generally be fine-tuned features of interaction with host factors.
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Dates et versions

hal-03009810 , version 1 (17-11-2020)

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Sonia Fieulaine, Martin D Witte, Christopher S Theile, Maya Ayach, Hidde L Ploegh, et al.. Turnip yellow mosaic virus protease binds ubiquitin suboptimally to fine-tune its deubiquitinase activity. Journal of Biological Chemistry, 2020, 295, pp.13769 - 13783. ⟨10.1074/jbc.ra120.014628⟩. ⟨hal-03009810⟩
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