Optimization of an ethanol/water-based sinapine extraction from mustard bran using Response Surface Methodology

Valentin Reungoat (1, 2) , Morvan Gaudin (1) , Amandine Flourat (1) , Emilie Isidore (1) , Louis M.M. Mouterde (1) , Florent Allais (1) , Hélène Ducatel (2) , Irina Ioannou (1)

1 ABI - Agro-Biotechnologies Industrielles
2 EXTRACTIS - Centre de Valorisation des Glucides et des Produits Naturels

This study reports an optimization of the extraction of sinapine from industrial mustard bran using an ethanol/water mixture as a green solvent. Response Surface Methodology was used to determine the optimal operating conditions for the extraction process. The results show that to obtain a maximum yield of sinapine, the extraction process must be carried out at 75 °C with 70% ethanol and a ratio of 10 mL/gBDM. Under these conditions, the extraction kinetics have shown that equilibrium is reached for a concentration of 8.8 ± 0.1 mg/gBDM in 30 min. The mustard bran is exhausted to 95% sinapine after two successive extractions. The selectivity of the extraction process for sinapine could be increased by 20% using an ethanol concentration of 83%, the yield of sinapine is then 8.0 ± 0.1 mg/gBDM.

Domaines: Génie des procédés

Valentin Reungoat, Morvan Gaudin, Amandine Flourat, Emilie Isidore, Louis M.M. Mouterde, et al.. Optimization of an ethanol/water-based sinapine extraction from mustard bran using Response Surface Methodology. Food and Bioproducts Processing, 2020, 122, pp.322-331. ⟨10.1016/j.fbp.2020.06.001⟩. ⟨hal-02893579⟩