Sequential time-kill: a simple experimental trick to discriminate between PK/PD models with distinct heterogeneous sub-populations versus homogenous population with adaptive resistance

Alexia Chauzy (1, 2) , H Ih (1, 2, 3) , M Jacobs (1, 2) , S Marchand (1, 2, 4) , N Grégoire (1, 2, 4) , W. Couet (4, 1, 2) , J. Buyck (1, 2)

1 PHAR [Poitiers] - Pharmacologie des anti-infectieux
2 Université de Poitiers - Faculté de Médecine et de Pharmacie
3 Tanjungpura University
4 CHU de Poitiers [La Milétrie] - Centre hospitalier universitaire de Poitiers = Poitiers University Hospital

Experiments were conducted with polymyxin B and two Klebsiella pneumonia isogenic strains (the wild-type, KP_WT and its transconjugant carrying the mobile colistin resistance gene, KP_MCR-1), to demonstrate that conducting 2 consecutive time-kill experiments, referred as sequential TK, represents a simple approach to discriminate between PK/PD models with two heterogeneous sub-populations (S/R) or adaptive resistance (AR).

Domaines: Sciences du Vivant [q-bio]; Sciences pharmaceutiques; Pharmacologie

Alexia Chauzy, H Ih, M Jacobs, S Marchand, N Grégoire, et al.. Sequential time-kill: a simple experimental trick to discriminate between PK/PD models with distinct heterogeneous sub-populations versus homogenous population with adaptive resistance. Antimicrobial Agents and Chemotherapy, 2020, ⟨10.1128/AAC.00788-20⟩. ⟨hal-02888859⟩