Production of formate from CO 2 gas under ambient conditions: towards flow-through enzyme reactors

Mohamed Baccour (1) , Alexandra Lamotte (1, 2) , Kento Sakai (3) , Eric Dubreucq (3) , Ahmad Mehdi (1) , Kenji Kano (3) , Anne Galarneau (1) , Jullien Drone (1) , Nicolas Brun (1)

1 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
2 Kyoto University
3 UMR IATE - Ingénierie des Agro-polymères et Technologies Émergentes

A flow-through bi-enzymatic reactor was developed to produce formate from carbon dioxide gas (CO2). The reaction operates under ambient conditions without hydrogen gas (H2) and involves a high performance formate dehydrogenase from Methylobacterium extorquens AM1 (MeFoDH1). The tandem reaction features in situ regeneration of the nicotinamide adenine dinucleotide (1,4-NADH) cofactor performed by a highly active mutant of the phosphite dehydrogenase from Pseudomonas stutzeri, without any side reaction. One of the highest turnover numbers reported to date in the literature was reached (6700) giving a high space-time yield of 0.6 mmolformate min-1 L-1reactor

Domaines: Ingénierie des aliments; Matériaux

Mohamed Baccour, Alexandra Lamotte, Kento Sakai, Eric Dubreucq, Ahmad Mehdi, et al.. Production of formate from CO 2 gas under ambient conditions: towards flow-through enzyme reactors. Green Chemistry, 2020, 22 (12), pp.3727-3733. ⟨10.1039/D0GC00952K⟩. ⟨hal-02884322⟩