Independent Emergence of the Plasmodium falciparum Kelch Propeller Domain Mutant Allele C580Y in Guyana

Stella M Chenet (1) , Sheila Akinyi Okoth (1) , Curtis S Huber (1) , Javin Chandrabose , Naomi W Lucchi (1) , Eldin Talundzic (1) , Nicolas Ceron , Lise Musset (2, 3, 4, 5) , Karanchand Krishnalall , Alexandre Macedo de Oliveira (1) , Meera Venkatesan , Reyaud Rahman , Venkatachalam Udhayakumar (1) , John W Barnwell (1)

1 DPDM - Division of Parasitic Diseases and Malaria [Atlanta, GA, États-Unis]
2 CNR - laboratoire associé - Centre National de Référence du Paludisme [Cayenne, Guyane française]
3 Laboratoire de Parasitologie [Cayenne, Guyane française]
4 Institut Pasteur de la Guyane
5 CCOMS - Centre Collaborateur OMS pour la surveillance de la résistance aux antipaludiques [Cayenne, Guyane française]

Suspected artemisinin resistance in Plasmodium falciparum can be explored by examining polymorphisms in the Kelch (PfK13) propeller domain. Sequencing of PfK13 and other gene resistance markers was performed on 98 samples from Guyana. Five of these samples carried the C580Y allele in the PfK13 propeller domain, with flanking microsatellite profiles different from those observed in Southeast Asia. These molecular data demonstrate independent emergence of the C580Y K13 mutant allele in Guyana, where resistance alleles to previously used drugs are fixed. Therefore, in Guyana and neighboring countries , continued molecular surveillance and periodic assessment of the therapeutic efficacy of artemisinin-based combination therapy are warranted.

Mots clés: malaria; South America; artemisinin resistance; K13; Plasmodium falciparum; Guyana; ACT

Domaines: Parasitologie; Génétique des populations [q-bio.PE]

Stella M Chenet, Sheila Akinyi Okoth, Curtis S Huber, Javin Chandrabose, Naomi W Lucchi, et al.. Independent Emergence of the Plasmodium falciparum Kelch Propeller Domain Mutant Allele C580Y in Guyana. Journal of Infectious Diseases, 2016, 213 (9), pp.1472-1475. ⟨10.1093/infdis/jiv752⟩. ⟨hal-02862975⟩