Regulation of Streptococcus pneumoniae clp genes and their role in competence development and stress survival - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Bacteriology Année : 2001

Regulation of Streptococcus pneumoniae clp genes and their role in competence development and stress survival

Résumé

In vitro mariner transposon mutagenesis of Streptococcus pneumoniae chromosomal DNA was used to isolate regulatory mutants affecting expression of the comCDE operon, encoding the peptide quorum-sensing two-component signal transduction system controlling competence development. A transposon insertion leading to increased comC expression was found to lie directly upstream from the S. pneumoniae clpP gene, encoding the proteolytic subunit of the Clp ATP-dependent protease, whose expression in Bacillus subtilis is controlled by the CtsR repressor. In order to examine clp gene regulation in S. pneumoniae, a detailed analysis of the complete genome sequence was performed, indicating that there are five likely CtsR-binding sites located upstream from the clpE, clpP, and clpL genes and the ctsR-clpC and groESL operons. The S. pneumoniae ctsR gene was cloned under the control of an inducible promoter and used to demonstrate regulation of the S. pneumoniae clpP and clpE genes and the clpC and groESL operons by using B. subtilis as a heterologous host. The CtsR protein of S. pneumoniae was purified and shown to bind specifically to the clpP, clpC, clpE, and groESL regulatory regions. S. pneumoniae Delta ctsR, Delta clpP, Delta clpC, and Delta clpE mutants were constructed by gene deletion/replacement. ClpP was shown to act as a negative regulator, preventing competence gene expression under inappropriate conditions. Phenotypic analyses also indicated that ClpP and ClpE are both required for thermotolerance. Contrary to a previous report, we found that ClpC does not play a major role in competence development, autolysis, pneumolysin production, or growth at high temperature of S. pneumoniae.
Fichier principal
Vignette du fichier
02-Chastanet-ClpP competence strep-01_1.pdf (1.05 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02683196 , version 1 (01-06-2020)

Identifiants

Citer

Arnaud Chastanet, Marc Prudhomme, Jean Pierre Claverys, Tarek Msadek. Regulation of Streptococcus pneumoniae clp genes and their role in competence development and stress survival. Journal of Bacteriology, 2001, 183 (24), pp.7295-7307. ⟨10.1128/JB.183.24.7295-7307.2001⟩. ⟨hal-02683196⟩
50 Consultations
90 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More