Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73

Catherine Duport (1) , S. Thomassin (1) , G. Bourel (1) , P. Schmitt (1)

1 SQPOV - Sécurité et Qualité des Produits d'Origine Végétale

Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1–0.2 h–1) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h–1, the presence of 90 mM glucose resulted in inhibition of HBL production. Glucose was found to repress HBL induction at the mRNA level, indicating the potential involvement of catabolite repression in the regulation of HBL. Based on these data, it is suggested that growth rate could be an effector of catabolite regulation of HBL.

Mots clés: RT-PCR

Domaines: Ingénierie des aliments; Génie des procédés; Sciences du Vivant [q-bio]

Catherine Duport, S. Thomassin, G. Bourel, P. Schmitt. Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73. Archives of Microbiology, 2004, 182 (1), pp.90-95. ⟨10.1007/s00203-004-0688-y⟩. ⟨hal-02681797⟩