Isolation and characterization of microsatellite loci from chub Leuciscus cephalus (Pisces : Cyprinidae)

Valerie Larno (1) , Sophie Launey (2) , Alain Devaux (3, 4) , Jean Laroche (5)

1 ESTP - Ecole Spéciale des Travaux Publics du Bâtiment et de l'Industrie
2 UGP - Unité de recherche Génétique des Poissons
3 DEPT EFPA - Département Ecologie des Forêts, Prairies et milieux Aquatiques
4 Laboratoire des Sciences de l’Environnement
5 UBO - Université de Brest

Twelve microsatellite markers were isolated from chub Leuciscus cephalus Linné, 1766 (Cyprinidae), a freshwater fish widely distributed in Europe. We assessed the level of genetic diversity for these loci in 24 individuals sampled in the Rhone river watershed. Nine loci were polymorphic, displaying from two to 13 alleles per locus with expected heterozygosity ranging from 0.160 to 0.887. Simultaneous migration of different polymerase chain reaction products was developed for routine analysis of L. cephalus populations

Mots clés: LEUCISCUS CEPHALUS; STRUTURE POPULATION

Domaines: Ecologie, Environnement

Valerie Larno, Sophie Launey, Alain Devaux, Jean Laroche. Isolation and characterization of microsatellite loci from chub Leuciscus cephalus (Pisces : Cyprinidae). Molecular Ecology Notes, 2005, 5 (4), pp.752-754. ⟨10.1111/j.1471-8286.2005.01052.x⟩. ⟨hal-02679522⟩