Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium

Li-Jun Ma (1) , Charlotte van Der Does (2) , Katherine A. Borkovich (3) , Jeffrey J. Coleman (4) , Marie-José Daboussi (5) , Antonio Di Pietro (6) , Marie Dufresne (5) , Michael Freitag (7) , Manfred Grabherr (1) , Bernard Henrissat (8) , Petra M. Houterman (2) , Seogchan Kang (9) , Won-Bo Shim (10) , Charles Woloshuk (11) , Xiaohui Xie (12) , Jin-Rong Xu (11) , John Antoniw (13) , Scott E. Baker (14) , Burton H. Bluhm (11) , Andrew Breakspear (15) , Daren W. Brown (16) , Robert A. E. Butchko (16) , Sinead Chapman (1) , Richard Coulson (17) , Pedro M Coutinho (8) , Etienne Danchin (18) , Andrew Diener (19) , Liane R. Gale (20) , Donald M. Gardiner (21) , Stephen Goff (4) , Kim E. Hammond-Kosack (13) , Karen Hilburn (20) , Aurelie Hua-Van (22) , Wilfried Jonkers (2) , Kemal Kazan (21) , Chinnappa D. Kodira (1) , Michael Koehrsen (1) , Lokesh Kumar (1) , Yong-Hwan Lee (23) , Liande Li (3) , John M. Manners (21) , Diego Miranda-Saavedra (24) , Mala Mukherjee (10) , Gyungsoon Park (3) , Jongsun Park (23) , Sook-Young Park (9) , Robert H. Proctor (16) , Aviv Regev (1) , Carmen Ruiz-Roldan (6) , Divya Sain (3) , Sharadha Sakthikumar (1) , Sean Sykes (1) , David C. Schwartz (25) , B. Gillian Turgeon (26) , Ilan Wapinski (1) , Olen Yoder (27) , Sarah Young (1) , Qiandong Zeng (1) , Shiguo Zhou (25) , James Galagan (1) , Christina A. Cuomo (1) , H. Corby Kistler (20) , Martijn Rep (2)

1 BROAD INSTITUTE - Broad Institute of MIT and Harvard
2 VU - Vrije Universiteit Amsterdam [Amsterdam]
3 UC Riverside - University of California [Riverside]
4 University of Arizona
5 IGM - Institut de génétique et microbiologie [Orsay]
6 Universidad de Córdoba = University of Córdoba [Córdoba]
7 OSU - Oregon State University
8 AFMB - Architecture et fonction des Macromolécules Biologiques - UMR 6098
9 Penn State - Pennsylvania State University
10 Texas A&M University System
11 Purdue University [West Lafayette]
12 UC Irvine - University of California [Irvine]
13 Rothamsted Research
14 PNNL - Pacific Northwest National Laboratory
15 UMN - University of Minnesota [Twin Cities]
16 USDA-ARS : Agricultural Research Service
17 European Molecular Biology Laboratory European Bioinformatics Institute
18 IBSV - Interactions Biotiques et Santé Végétale
19 UC Davis - University of California [Davis]
20 ARS
21 Queensland Bioscience Precinct
22 UP11 - Université Paris-Sud - Paris 11
23 SNU - Seoul National University [Seoul]
24 CAM - University of Cambridge [UK]
25 University of Wisconsin-Madison
26 Cornell University [New York]
27 Independent

Fusarium species are among the most important phytopathogenic and toxigenic fungi. To understand the molecular underpinnings of pathogenicity in the genus Fusarium, we compared the genomes of three phenotypically diverse species: Fusarium graminearum, Fusarium verticillioides and Fusarium oxysporum f. sp. lycopersici. Our analysis revealed lineage-specific (LS) genomic regions in F. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. LS regions are rich in transposons and genes with distinct evolutionary profiles but related to pathogenicity, indicative of horizontal acquisition. Experimentally, we demonstrate the transfer of two LS chromosomes between strains of F. oxysporum, converting a non-pathogenic strain into a pathogen. Transfer of LS chromosomes between otherwise genetically isolated strains explains the polyphyletic origin of host specificity and the emergence of new pathogenic lineages in F. oxysporum. These findings put the evolution of fungal pathogenicity into a new perspective

Mots clés: MAXIMUM-LIKELIHOOD; POLYMORPHISM; HOST PATHOGEN RELATIONSHIPS; PATHOGENICITY; DISEASE RESISTANCE; FUSARIUM GRAMINEARUM; FUSARIUM VERTICILLIODES; FUSARIUM OXYSPORUM

Mots clés: RELATION HOTE-PARASITE

Domaines: Biologie cellulaire

Li-Jun Ma, Charlotte van Der Does, Katherine A. Borkovich, Jeffrey J. Coleman, Marie-José Daboussi, et al.. Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature, 2010, 464 (7287), pp.367-373. ⟨10.1038/nature08850⟩. ⟨hal-02662964⟩