Skip to Main content Skip to Navigation
Journal articles

Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle

Abstract : Ovarian follicle provides a favorable environment for enclosed oocytes, which acquire their competence in supporting embryo development in tight communications with somatic follicular cells and follicular fluid (FF). Although steroidogenesis in theca (TH) and granulosa cells (GC) is largely studied, and the molecular mechanisms of fatty acid (FA) metabolism in cumulus cells (CC) and oocytes are emerging, little data is available regarding lipid metabolism regulation within ovarian follicles. In this study, we investigated lipid composition and the transcriptional regulation of FA metabolism in 3⁻8 mm ovarian follicles in bovine. Using liquid chromatography and mass spectrometry (MS), 438 and 439 lipids were identified in FF and follicular cells, respectively. From the MALDI-TOF MS lipid fingerprints of FF, TH, GC, CC, and oocytes, and the MS imaging of ovarian sections, we identified 197 peaks and determined more abundant lipids in each compartment. Transcriptomics revealed lipid metabolism-related genes, which were expressed constitutively or more specifically in TH, GC, CC, or oocytes. Coupled with differential lipid composition, these data suggest that the ovarian follicle contains the metabolic machinery that is potentially capable of metabolizing FA from nutrient uptake, degrading and producing lipoproteins, performing de novo lipogenesis, and accumulating lipid reserves, thus assuring oocyte energy supply, membrane synthesis, and lipid-mediated signaling to maintain follicular homeostasis.
Document type :
Journal articles
Complete list of metadata

Cited literature [107 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02628741
Contributor : Migration Prodinra <>
Submitted on : Tuesday, May 26, 2020 - 11:42:35 PM
Last modification on : Tuesday, September 21, 2021 - 4:18:08 PM

File

2018_Bertevello_int j mol sci_...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Priscila Bertevello, Ana-Paula Teixeira-Mechin, Alexandre Seyer, Anais Carvalho, Valérie Labas, et al.. Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle. International Journal of Molecular Sciences, MDPI, 2018, 19 (10), pp.3261. ⟨10.3390/ijms19103261⟩. ⟨hal-02628741⟩

Share

Metrics

Record views

88

Files downloads

269