Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication

Romain Forey (1) , Ana Poveda (2) , Sushma Sharma (3) , Antoine Barthe (1) , Ismaël Padioleau (1) , Claire Renard (1) , Robin Lambert (1) , Magdalena Skrzypczak (4) , Krzysztof Ginalski (4) , Armelle Lengronne (1) , Andrei Chabes (3) , Benjamin Pardo (1) , Philippe Pasero (1)

1 IGH - Institut de génétique humaine
2 Centro Internacional de Zoonosis, Facultad de Ciencias Químicas, Facultad de Medicina Veterinaria , Universidad Central del Ecuador , Quito , Ecuador.
3 Department of Medical Biochemistry and Biophysics and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå
4 Laboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of Warsaw, 02-089 Warsaw, Poland

Highlights d The firing of early replication origins activates Mec1 ATR d The upregulation of dNTP pools prevents Mec1 ATR activation d Mec1 ATR triggers dNTP synthesis to complete S phase d Rad53 prevents fork collapse during dNTP shortage

Domaines: Biologie moléculaire; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Romain Forey, Ana Poveda, Sushma Sharma, Antoine Barthe, Ismaël Padioleau, et al.. Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication. Molecular Cell, 2020, 78 (3), pp.396-410.e4. ⟨10.1016/j.molcel.2020.02.021⟩. ⟨hal-02612438⟩