Heterogeneous mixture of amniotic cells is likely a better source of stem cells than adipose tissue

Diana Kitala , Agnieszka Klama-Baryła , Marcelina Misiuga , Wojciech Łabuś , Małgorzata Kraut , Michał Szapski , Marta Lesiak , Daniel Krakowian , Aleksander L Sieroń , Marek J Łos (1, 2) , Marek Kucharzewski

1 CBM - Centre de biophysique moléculaire
2 Le Studium Loire Valley Institute for Advanced Studies, 45000 Orléans, France

Stem cells are increasingly being used in the course of burn treatment. As several different types of stem cells are available for the purposes, it is important to chose the most efficient and the most practicable stem cell type. The aim of this study was to compare the potential of heterogeneous amnion cell mixture with the presently used standard therapy, the adipose tissue-derived stem cells. The placenta was collected during a Cesarean section procedure. Adipose tissue tissue-derived cells were isolated using the Cytori’s Celution® System. Cells were tested for fulfillment of the minimum criteria for stem cells. The efficiency of cell cultures was tested by an analysis of population doubling, cell proliferation, cell cycle and cell migration. Amniotic cells presented a higher ability for differentiation to chondrocytes and osteocytes than adipose-derived regenerative cells but a lower ability for differentiation toward adipocytes. Additionally, in vitro experiments have demonstrated a higher applicability of amniotic cells than adipose tissue-derived stem cells. Amniotic cells show several advantages: easy access to placenta, low costs and a lack of ethical dilemmas related to stem cell harvesting. The main disadvantage is, however, their availability, as isogenic treatment would only be possible for women around children-bearing age, unless personalized banks for amniotic cells would be established.

Domaines: Immunologie

Diana Kitala, Agnieszka Klama-Baryła, Marcelina Misiuga, Wojciech Łabuś, Małgorzata Kraut, et al.. Heterogeneous mixture of amniotic cells is likely a better source of stem cells than adipose tissue. Archivum Immunologiae et Therapiae Experimentalis, 2019, 67 (3), pp.189-196. ⟨10.1007/s00005-019-00538-5⟩. ⟨hal-02572164⟩