Performance comparison with different methods for ethanol/O2 biofuel cell based on NAD+ cofactor immobilized and activated by two types of carbon nanoparticles

Djamel Selloum , Vincent Techer , Abdellah Henni , Sophie Tingry , Marc Cretin , Christophe Innocent (1)

1 IEM - Institut Européen des membranes

Biofuel cells are an attractive alternative to conventional fuel cells, because they use biological catalysts. We report in this article the construction of an ethanol / O2 enzymatic biofuel cell. In the first time, the performance comparison with different methods for Ethanol/O2 biofuel cell has been study. Alcohol dehydrogenase, a Nicotinamide Adenine Dinucleotide (NAD) dependant enzyme, is immobilized with NAD + , diaphorase and vitamin K3 (VK3) on the electrode. The oxygen is reduced at the cathode with laccase and 2,2'azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS).The performances of the electrodes are improved thanks to addition of carbon powder (KS6 and Super P ® carbon). The benefit of the carbon particles with higher surface porosity was explained by the high porous structure that offered a closer proximity to the reactive species and improved diffusion of ethanol and oxygen within the enzyme films. Efficiency of immobilization of NAD + cofactor has been also demonstrated. We have shown the effect of multienzymatic reaction at the anode on the storage and operational stability. A high open circuit potential has been recorded 1.02V, demonstrating the benefit of NAD + immobilization with maximal power density of 300 µW.cm-2. Chronoamperometric measurements show a current density of 90 µA.cm-2 during 20 hours demonstrating the operational stability of the ethanol biofuel cell based on NAD + cofactor immobilization.

Mots clés: bioelectrochemistry; enzymatic biofuel cell; carbon particles; alcohol dehydrogenase; diaphorase

Domaines: Chimie

Djamel Selloum, Vincent Techer, Abdellah Henni, Sophie Tingry, Marc Cretin, et al.. Performance comparison with different methods for ethanol/O2 biofuel cell based on NAD+ cofactor immobilized and activated by two types of carbon nanoparticles. Journal of Solid State Electrochemistry, 2020, 24 (3), pp.623-631. ⟨10.1007/s10008-020-04506-4⟩. ⟨hal-02570184⟩