Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2019

Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes

Résumé

Studies of Plasmodium vivax gene expression are complicated by the lack of in vitro culture system and the difficulties associated with studying clinical infections that often contain multiple clones and a mixture of parasite stages. Here, we characterize the transcriptomes of P. vivax parasites from 26 malaria patients. We show that most parasite mRNAs derive from trophozoites and that the asynchronicity of P. vivax infections is therefore unlikely to confound gene expression studies. Analyses of gametocyte genes reveal two distinct clusters of co-regulated genes, suggesting that male and female gametocytes are independently regulated. Finally, we analyze gene expression changes induced by chloroquine and show that this antimalarial drug efficiently eliminates most P. vivax parasite stages but, in contrast to P. falciparum, does not affect trophozoites.

Domaines

Parasitologie

Dates et versions

hal-02558688 , version 1 (29-04-2020)

Identifiants

Citer

Adam Kim, Jean Popovici, Didier Ménard, David Serre. Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes. Nature Communications, 2019, 10 (1), pp.371. ⟨10.1038/s41467-019-08312-z⟩. ⟨hal-02558688⟩

Collections

RIIP RIIP_CAMBODGE
29 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More