Identification of fungal fossils and novel azaphilone pigments in ancient carbonised specimens of Hypoxylon fragiforme from forest soils of Châtillon-sur-Seine (Burgundy)

Frank Surup (1, 2) , Abolfazl Narmani (1, 3) , Lucile Wendt (1, 2) , Sebastian Pfütze (1, 2) , Robin Kretz (1) , Kevin Becker (1, 2) , Clément Menbrivès (4) , Alain Giosa (4) , Elliott Michelle (4, 5) , Christophe Petit (4, 5) , Manfred Rohde (1) , Marc Stadler (1, 2)

1 HZI - Helmholtz-Zentrum für Infektionsforschung GmbH
2 DZIF - German Centre for Infection Research
3 University of Tabriz [Tabriz]
4 Archéologies environnementales
5 UP1 UFR03 - Université Paris 1 Panthéon-Sorbonne - UFR Histoire de l'art et archéologie

Fungal stromata were recently discovered in association with charcoal and burnt soil aggregates during an archaeological survey in the Châtillon-sur-Seine forest massif. The wood and soil in the samples were dated to the medieval period (between 738 and 1411 AD). Light microscopy and scanning electron microscopy revealed that a few of the stromatal fragments still contained ascospores. Their macromorphological characters were described and secondary metabolite profiles were generated using high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS). The combination of these two data lines then allowed species identification. Most of the fragments were assigned to Hypoxylon fragiforme, the type species of the Hypoxylaceae (Xylariales). Two further species whose stromata grew on the fossil charcoal could be tentatively identified as Jackrogersella cohaerens and (more tentatively) as Hypoxylon vogesiacum. These three species are still commonly encountered in the forests of Central Europe today. Furthermore, the HPLC-HRMS data of H. fragiforme suggested the presence of unknown azaphilone dimers and of further new pigments. These archaeological compounds were compared to fresh stromata of H. fragiforme collected in Germany and subjected to the same analytical protocol. While the major components in both samples were identified as the known mitorubrin type azaphilones and orsellinic acid, the chemical structures of seven novel complex azaphilone pigments, for which we propose the trivial names rutilins C-D and fragirubrins A-E, were elucidated using spectral methods (NMR and CD spectroscopy, high resolution mass spectrometry). It appears that these pigments had indeed persisted for millennia in the fossil stromata.

Mots clés: Biodiversity; Chemotaxonomy; Phylogeny; Sordariomycetes; Xylariales; Structure elucidation

Domaines: Sciences de l'Homme et Société; Archéologie et Préhistoire

Frank Surup, Abolfazl Narmani, Lucile Wendt, Sebastian Pfütze, Robin Kretz, et al.. Identification of fungal fossils and novel azaphilone pigments in ancient carbonised specimens of Hypoxylon fragiforme from forest soils of Châtillon-sur-Seine (Burgundy). Fungal Diversity, 2018, 92, pp.345-356. ⟨hal-02555602⟩