Skip to Main content Skip to Navigation
Journal articles

Native Collagen: Electrospinning of Pure, Cross-Linker-Free, Self-Supported Membrane

Abstract : Rebuilding biological environments is crucial when facing the challenges of fundamental and biomedical research. Thus, preserving the native state of biomolecules is essential. We use electrospinning (ES), which is an extremely promising method for the preparation of fibrillar membranes to mimic the ECM of native tissues. Here, we report for the first time (1) the ES of pure and native collagen into a self-supported membrane in absence of crosslinker and polymer support, (2) the preservation of the membrane integrity in hydrated media in absence of crosslinker and (3) the preservation of the native molecular structure and recovery of the hierarchical assembly of collagen. We use a multiscale approach to characterize collagen native structure at the molecular level using circular dichroism, and to investigate collagen hierarchical organization within the self-supported membrane using a combination of multiphoton and electron microscopies. Finally, we show that the membranes are perfectly suited for cell adhesion and spreading, making them very promising candidates for the development of biomaterials and finding applications in biomedical research.
Document type :
Journal articles
Complete list of metadatas

Cited literature [59 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02541707
Contributor : Carole Aimé <>
Submitted on : Tuesday, April 14, 2020 - 9:57:29 AM
Last modification on : Thursday, June 11, 2020 - 1:16:03 PM

File

HAL-AIME-Manuscript.pdf
Files produced by the author(s)

Identifiers

Citation

Dounia Dems, Julien Rodrigues da Silva, Christophe Hélary, Frank Wien, Marion Marchand, et al.. Native Collagen: Electrospinning of Pure, Cross-Linker-Free, Self-Supported Membrane. ACS Applied Bio Materials, ACS Publications, 2020, ⟨10.1021/acsabm.0c00006⟩. ⟨hal-02541707⟩

Share

Metrics

Record views

60

Files downloads

83