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, Département de chirurgie digestive et de l'urgence, Centre Hospitalier Grenoble-Alpes, vol.38000
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, Laboratoire d'anatomie des Alpes françaises (LADAF)
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, Département d'anatomopathologie et cytologie, Centre Hospitalier Grenoble-Alpes, vol.38000
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, INSERM U1209/CNRS UMR 5309
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, 34093 Montpellier cedex 05, France. Tel: +33 (0)411759697. Email address: benjamin.nottelet@umontpellier.fr Content of supplementary data: S1-Synthetic procedures for PCL-TIB
Samples implanted in rats, CT imaging tri-dimensional visualization S4-Preliminary evaluation of blend cytocompatibility ,
Proliferation at 1, 3 and 7 days of L929 murine fibroblasts on the radiopaque PCL-TIB/PLA50-b-PEG-b-PLA50 (2,5 wt% iodine) blends compared to pure PLA50-b-PEG-b-PLA50 (0 wt% iodine) and TCPS (Tissue Culture ,
PCL (35 mmol of CL unit, 4 g) was dissolved in anhydrous THF (400 mL) in a fournecked reactor equipped with a mechanical stirrer and kept at -50 °C under argon atmosphere ,
, A solution of LDA (0.5 eq./CL unit, 8.8 mL) was injected with a syringe through a septum and the mixture was kept at -50 °C under stirring for 30 min
, 6 g) was added, the mixture was kept under stirring for 30 min at a temperature raising from -50 °C to -30°C. The reaction was then stopped by addition of a saturated solution of NH4Cl (300 mL) and pH was adjusted to 7 with 1M HCl. The polymer was extracted with dichloromethane (3 × 150 mL). The combined organic phases were washed three times with distilled water (3 × 150 mL), dried on anhydrous MgSO4 and filtered. After partial solvent evaporation under reduced pressure, the polymer was recovered by two precipitations in cold methanol and dried overnight under vacuum. The polymer PCL-TIB11
, 300 MHz; CDCl3) : ? (ppm) = 8.3 and 7.4-7.6 (2H, CH), 4.0 (2H, CH2-O), 2.3 (2H, C(O)CH2), 1.6 (4H, C(O)CH2-CH2-CH2-CH2-CH2-O), 1.4 (2H, C(O)CH2-CH2-CH2-CH2-CH2-O). n ???? = 24 000 g.mol -1 , Ð =, vol.1