Characterization of the dentin microstructural components: a FIB-SEM analysis.

Ursule Muendi , Thomas Reiss (1) , Elisabeth Dursun (2) , Elsa Vennat (2, 1)

1 MSSMat - Laboratoire de mécanique des sols, structures et matériaux
2 URB2i - EA 4462 - Unité de Recherche Biomatériaux Innovants et Interfaces

Introduction Dentin is the main tissue of the tooth. It is composed of peritubular dentin (PTD), intertubular dentin (ITD) and tubules (T). This microstructure has been mainly studied in 2D using optical microscopy [1], Scanning Electronic Microscopy (SEM) [2] and Atomic Force Microscopy (AFM) [3]. Among the few 3D studies of its architecture, tubules have been observed and quantified within the first 350 µm from the dentin-enamel junction (DEJ) using confocal laser scanning microscopy (CLSM) by Vennat et al. [4] It has been shown that the tubules have a complex geometry with many channels connecting them. Even in the localized area near DEJ, dentin shows properties gradients. In order to widen the knowledge of the dentinal microstructure, there is a need to investigate the three components of dentin (PTD, ITD and T) within the whole dentinal tissue. As FIB-SEM allows imaging the three components of dentin in 3D, a FIB-SEM study has been led in order to investigate their organization in 3D. FIB-SEM has already been used on dentin [5], [6] but we argue that more parameters can be extracted from the data obtained, especially concerning the PTD and ITD and the objective of this paper is to prove it.

Domaines: Biomécanique [physics.med-ph]

Ursule Muendi, Thomas Reiss, Elisabeth Dursun, Elsa Vennat. Characterization of the dentin microstructural components: a FIB-SEM analysis.. 13th International Conference on Advances in Experimental Mechanics (BSSM 2018), 2018, Southampton, United Kingdom. ⟨hal-02513327⟩