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Murine genetic background overcomes gut microbiota changes to explain metabolic response to high-fat diet

Abstract : Interactions of diet, gut microbiota, and host genetics play essential roles in the development of metabolic diseases. A/J and C57BL/6J (C57) are two mouse strains known to display different susceptibilities to metabolic disorders. In this context, we analyzed gut microbiota composition in A/J and C57 mice, and assessed its responses to high-fat diet (HFD) and antibiotic (AB) treatment. We also exchanged the gut microbiota between the two strains following AB treatment to evaluate its impact on the metabolism. We showed that A/J and C57 mice have different microbiome structure and composition at baseline. Moreover, A/J and C57 microbiomes responded differently to HFD and AB treatments. Exchange of the gut microbiota between the two strains was successful as recipients’ microbiota resembled donor-strain microbiota. Seven weeks after inoculation, the differences between recipients persisted and were still closer from the donor-strain microbiota. Despite effective microbiota transplants, the response to HFD was not markedly modified in C57 and A/J mice. Particularly, body weight gain and glucose intolerance in response to HFD remained different in the two mouse strains whatever the changes in microbiome composition. This indicated that genetic background has a much stronger impact on metabolic responses to HFD than gut microbiome composition. View
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https://hal.archives-ouvertes.fr/hal-02503303
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Submitted on : Monday, March 9, 2020 - 8:11:13 PM
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Zahra Safari, Aurelia Bruneau, Magali Monnoye, Mahendra Mariadassou, Catherine Philippe, et al.. Murine genetic background overcomes gut microbiota changes to explain metabolic response to high-fat diet. Nutrients, MDPI, 2020, 12 (2), ⟨10.3390/nu12020287⟩. ⟨hal-02503303⟩

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