Skip to Main content Skip to Navigation
Journal articles

Murine genetic background overcomes gut microbiota changes to explain metabolic response to high-fat diet

Abstract : Interactions of diet, gut microbiota, and host genetics play essential roles in the development of metabolic diseases. A/J and C57BL/6J (C57) are two mouse strains known to display different susceptibilities to metabolic disorders. In this context, we analyzed gut microbiota composition in A/J and C57 mice, and assessed its responses to high-fat diet (HFD) and antibiotic (AB) treatment. We also exchanged the gut microbiota between the two strains following AB treatment to evaluate its impact on the metabolism. We showed that A/J and C57 mice have different microbiome structure and composition at baseline. Moreover, A/J and C57 microbiomes responded differently to HFD and AB treatments. Exchange of the gut microbiota between the two strains was successful as recipients’ microbiota resembled donor-strain microbiota. Seven weeks after inoculation, the differences between recipients persisted and were still closer from the donor-strain microbiota. Despite effective microbiota transplants, the response to HFD was not markedly modified in C57 and A/J mice. Particularly, body weight gain and glucose intolerance in response to HFD remained different in the two mouse strains whatever the changes in microbiome composition. This indicated that genetic background has a much stronger impact on metabolic responses to HFD than gut microbiome composition. View
Document type :
Journal articles
Complete list of metadata

Cited literature [60 references]  Display  Hide  Download
Contributor : Archive Ouverte Prodinra <>
Submitted on : Monday, March 9, 2020 - 8:11:13 PM
Last modification on : Tuesday, June 15, 2021 - 2:57:32 PM
Long-term archiving on: : Wednesday, June 10, 2020 - 5:07:02 PM


Explicit agreement for this submission


Distributed under a Creative Commons Attribution 4.0 International License



Zahra Safari, Aurelia Bruneau, Magali Monnoye, Mahendra Mariadassou, Catherine Philippe, et al.. Murine genetic background overcomes gut microbiota changes to explain metabolic response to high-fat diet. Nutrients, MDPI, 2020, 12 (2), ⟨10.3390/nu12020287⟩. ⟨hal-02503303⟩



Record views


Files downloads