Positive quantitative PCR detecting Fusarium solani in a case of mixed invasive fungal disease due to Mucorales and Fusarium solani

Positive quantitative PCR detecting Fusarium solani in a case of mixed invasive fungal disease due to Mucorales and Fusarium solani.

Anne-Pauline Bellanger (1, 2) , Steffi Rocchi (1, 2) , Ana Berceanu (3) , Emeline Scherer (1, 2) , Fabrice Larosa (4) , Laurence Millon (1, 2)

1 LCE - Laboratoire Chrono-environnement (UMR 6249)
2 Service de parasitologie et mycologie [CHRU de Besançon]
3 Service d'hématologie
4 Service d'Hématologie Clinique (CHU de Dijon)

We present a case of invasive fungal co-infection in a young patient treated for an acute myeloid leukemia and having undergone a twice-haploid matched unrelated donor hematopoietic stem cell transplantation (HSCT) with two different donors. A mucormycosis diagnosis was made shortly after the patient's admission using imagery and specific Mucorales qPCR which was treated with liposomal amphotericin B and posaconazole. Twenty days later, a blood culture was positive for Fusarium solani, and disseminated cutaneous lesions appeared. The antifungal treatment was changed to liposomal amphotericin B and voriconazole. Thanks to a complete hematological reconstitution and despite a co-infection with two aggressive filamentous opportunistic fungi, the patient recovered. We took advantage of this clinical case to test a specific Fusarium solani qPCR, which proved to be promising when performed retrospectively on some of the patient samples.

Domaines: Santé

Anne-Pauline Bellanger, Steffi Rocchi, Ana Berceanu, Emeline Scherer, Fabrice Larosa, et al.. Positive quantitative PCR detecting Fusarium solani in a case of mixed invasive fungal disease due to Mucorales and Fusarium solani. Bone Marrow Transplantation, 2020, ⟨10.1038/s41409-020-0819-3⟩. ⟨hal-02489421⟩