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, Supplementary Information 3: Calcium signaling
, Figure S 3: Absence of calcium signaling triggered by flow supports absence of mechanotransduction in flow mechanotaxis. Calcium signaling versus onset of flow (left) or addition of ionomycin (right) in cells loaded with Oregon Green 488 BAPTA-1 and crawling on ICAM-1 -top) or VCAM-1 (bottom) substrates
, Supplementary movies (see, Biophysical Journal, vol.2020, issue.3, pp.565-577
, Movie S1: T cell motility phenotype under flow (8 dyn.cm ---2 ) on ICAM-1 (left) and VCAM-1 (right) substrates. Bright-field images with magnification x10
, Movie S2: T cell motility phenotype under flow (8 dyn.cm -2 ) on mixed ICAM-1/ VCAM-1 substrates. Transmission images with magnification x10
, cm -2 ) on mixed 50 % ICAM-1 / 50 % VCAM-1 substrate. Transmission (left) and RICM (right) images with magnification x63, Movie S3: T cell motility phenotype under flow (4 dyn
Image sequence of lymphocytes crawling on VCAM-1 coated glass substrate at a shear stress of 8 dyn ,