NEURONAL CULTURE AT SINGLE CELL LEVEL FOR CREATION OF BIOLOGICAL NEURONAL NETWORK

Stephany Mai Nishikawa , Soo Hyeon Kim , Yoshiho Ikeuchi (1) , Timothée Levi (2) , Teruo Fujii (3)

1 IIS - Institute of Industrial Science
2 IMS - Laboratoire de l'intégration, du matériau au système
3 LIMMS - Laboratory for Integrated Micro Mechatronics Systems

To understand the electrophysiological dynamics of neurons, the use of micro-electrode array (MEA) is common because it is non-invasive and it allows long-term monitoring. However, as the culture are randomly arranged on fixated electrodes, it is not capable of recording electrophysiology at single cell level. Here, we present a fully transparent neuronal culture device capable of monitoring cells growth or activity at single scale level. To trap cells singularly, dielectrophoresis is used on a ITO glass coated with fluoropolymer component called CYTOP® to manipulate cells.

Domaines: Sciences de l'ingénieur [physics]

Stephany Mai Nishikawa, Soo Hyeon Kim, Yoshiho Ikeuchi, Timothée Levi, Teruo Fujii. NEURONAL CULTURE AT SINGLE CELL LEVEL FOR CREATION OF BIOLOGICAL NEURONAL NETWORK. MicroTAS, Oct 2019, Bale, Switzerland. ⟨hal-02484009⟩