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Poster communications

Étude de la maturité des porcelets en fin de gestation par une approche métabolomique multifluide

Abstract : Selection for prolificacy and lean growth in swine has been associated with a substantial increase in piglet mortality. The first 24-48 hours after birth represent the most critical period. A major determinant for early survival is piglet maturity at birth, which relies strongly on maturation during the last month of gestation. The objective of the current study was to compare progeny from Large White and Meishan sows to describe the metabolic status of their fetuses. These two breeds differ in piglet survival. Thirty-nine sows were inseminated with mixed semen and anesthetized at 90 or 110 days after conception (mean gestation: 114 days). Their fetuses (mean = 15.7 per sow) were quickly removed by caesarean section, and this study focused on plasmatic, urinary and amniotic liquid metabolomes (1H-NMR) of 507-604 fetuses. Raw 1D Bruker spectral data files were treated with ASICS, an R package available on Bioconductor. ASICS allowed for direct identification and quantification of ca. 90 metabolites from a library of 190 metabolites, with 63-68 metabolites per fluid, of which 39 were common to the three fluids. Mixed models were applied to the quantification results to explain differences between gestational stages and fetal genotypes. A total of 55 metabolites for plasma, 37 for urine and 46 for amniotic liquid were found to be differential (FDR < 0.05). The results provide new insights into biological pathways involved in piglet maturation. In particular, we found large differences in metabolite concentrations between the two stages of gestation and between the fetal genotypes for certain amino acids and sugars in plasma.
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Submitted on : Tuesday, June 2, 2020 - 8:07:42 PM
Last modification on : Friday, May 20, 2022 - 9:04:58 AM
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  • HAL Id : hal-02479994, version 1
  • PRODINRA : 493795

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Gaëlle Lefort, Nathalie Vialaneix, Hélène Quesnel, Marie-Christine Pere, Yvon Billon, et al.. Étude de la maturité des porcelets en fin de gestation par une approche métabolomique multifluide. 52. Journées de la Recherche Porcine, Feb 2020, Paris, France. IFIP - Institut du Porc, Journées de la Recherche Porcine en France, pp.411-412, 2020, 52èmes journées de la recherche porcine. ⟨hal-02479994⟩

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