Vascular bifurcation mapping with photoacoustic microscopy

Boudewijn van Der Sanden (1) , Olivier Hugon (2) , Mehdi Inglebert (2) , Olivier Jacquin (2) , Eric Lacot (2)

1 Inserm U836, équipe 7, Nanomédecine et cerveau
2 OPTIMA-LIPhy - OPTique et IMAgeries [Grenoble]

The early detection of microvascular changes in cancer diagnosis is needed in the clinic. A change in the vascular bifurcation density is a biomarker for the sprouting activity. Here, Optical-Resolution PhotoAcoustic Microscopy is used for quantitative vascular bifurcation mapping in 2D after the creation of Virtual Tubes out of Bifurcations. In stacks of OR-PAM images of the hemoglobin distribution, bifurcations become tubes and are selected by the 3D tubeness filter. These fast analyses will be compared to a classical approach and are easier to implement for functional analysis of the vascular bifurcation density in healthy and diseased tissues.

Domaines: Optique [physics.optics]

Boudewijn van Der Sanden, Olivier Hugon, Mehdi Inglebert, Olivier Jacquin, Eric Lacot. Vascular bifurcation mapping with photoacoustic microscopy. Biomedical optics express, 2020, 11 (3), pp.1298. ⟨10.1364/BOE.383583⟩. ⟨hal-02475499⟩