RNA Nanostructure Molecular Imaging

Olivier Pietrement (1) , Véronique Arluison (2) , Christophe Lavelle (3)

1 ICB - Laboratoire Interdisciplinaire Carnot de Bourgogne
2 MMB - LLB - Matière molle et biophysique
3 Régulation et dynamique des génomes

This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Biologie moléculaire

Olivier Pietrement, Véronique Arluison, Christophe Lavelle. RNA Nanostructure Molecular Imaging. RNA Spectroscopy, pp.319-327, 2020, ⟨10.1007/978-1-0716-0278-2⟩. ⟨hal-02467544⟩