Ozone Effects on Botrytis cinerea Conidia using a Bubble Column: Germination Inactivation and Membrane Phospholipids Oxidation

Marielle Pages (1, 2) , Didier Kleiber (1, 2) , Romain Pierron (1, 2) , Frédéric Violleau (1, 2)

1 CAI - Chimie Agro-Industrielle
2 INP - PURPAN - Ecole d'Ingénieurs de Purpan

Ozone treatments were applied on conidia aqueous suspensions in order to determine theminimal applied ozone dose to limit conidia germination and to observe the mechanismsinvolved in the spores inactivation. Conidia germination was significantly reduced, bubbling forat least 0.5 min as a gas with a minimal ozone concentration of 1 g.m−3. The applied ozone dosesinduce the membrane phospholipids oxidation, determined by the malondialdehyde quantifica-tion. Membrane phospholipids oxidation and inactivation rate are correlated. So, lipid peroxida-tion and consequently the alteration of the membrane integrity are involved in the antifungalaction of ozone.

Mots clés: Ozone; Botrytis cinerea; Bubble Column; Conidia; Germination; LipidPeroxidation

Domaines: Sciences agricoles

Marielle Pages, Didier Kleiber, Romain Pierron, Frédéric Violleau. Ozone Effects on Botrytis cinerea Conidia using a Bubble Column: Germination Inactivation and Membrane Phospholipids Oxidation. Ozone: Science and Engineering, 2016, 38 (1), pp.62-69. ⟨10.1080/01919512.2015.1074856⟩. ⟨hal-02459801⟩