Effect of engineered nanoparticles on vasomotor responses in rat intrapulmonary artery.

Arnaud Courtois (1) , Pascal Andujar (2, 3) , Yannick Ladeiro (1) , Thomas Ducret (1) , Françoise Rogerieux (4) , Ghislaine Lacroix (4) , Isabelle Baudrimont (1) , Christelle Guibert (1) , Etienne Roux (1) , Mireille Canal-Raffin (5, 6) , Patrick Brochard (5, 6) , Francelyne Marano (7, 8) , Roger Marthan (1, 6) , Bernard Muller (1)

1 Physiopathologie de la réactivite bronchique et vasculaire
2 IMRB - Institut Mondor de Recherche Biomédicale
3 Service de pneumologie et pathologie professionnelle
4 INERIS - Institut National de l'Environnement Industriel et des Risques
5 Laboratoire Santé Travail Environnement
6 CHU Bordeaux
7 Laboratoire de Cytophysiologie et Toxicologie Cellulaire
8 LPN - Laboratoire de physiopathologie de la nutrition

Pulmonary circulation could be one of the primary vascular targets of finest particles that can deeply penetrate into the lungs after inhalation. We investigated the effects of engineered nanoparticles on vasomotor responses of small intrapulmonary arteries using isometric tension measurements. Acute in vitro exposure to carbon nanoparticles (CNP) decreased, and in some case abolished, the vasomotor responses induced by several vasoactive agents, whereas acute exposure to titanium dioxide nanoparticles (TiO(2)NP) did not. This could be attributed to a decrease in the activity of those vasoactive agents (including PGF(2)(alpha), serotonin, endothelin-1 and acetylcholine), as suggested when they were exposed to CNP before being applied to arteries. Also, CNP decreased the contraction induced by 30 mM KCl, without decreasing its activity. After endoplasmic reticulum calcium stores depletion (by caffeine and thapsigargin), CaCl(2) addition induced a contraction, dependent on Store-Operated Calcium Channels that was not modified by acute CNP exposure. Further addition of 30 mM KCl elicited a contraction, originating from activation of Voltage-Operated Calcium Channels that was diminished by CNP. Contractile responses to PGF(2)(alpha) or KCl, and relaxation to acetylcholine were modified neither in pulmonary arteries exposed in vitro for prolonged time to CNP or TiO(2)NP, nor in those removed from rats intratracheally instilled with CNP or TiO(2)NP. In conclusion, prolonged in vitro or in vivo exposure to CNP or TiO(2)NP does not affect vasomotor responses of pulmonary arteries. However, acute exposure to CNP decreases contraction mediated by activation of Voltage-Operated, but not Store-Operated, Calcium Channels. Moreover, interaction of some vasoactive agents with CNP decreases their biological activity that might lead to misinterpretation of experimental data.

Domaines: Biochimie, Biologie Moléculaire

Arnaud Courtois, Pascal Andujar, Yannick Ladeiro, Thomas Ducret, Françoise Rogerieux, et al.. Effect of engineered nanoparticles on vasomotor responses in rat intrapulmonary artery.. Toxicology and Applied Pharmacology, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩. ⟨inserm-00495071⟩