Molecular Variability and Genetic Structure of IYMV in Burkina Faso

Moustapha Koala , Drissa Sérémé (1) , Florence Vignols (2) , Eric Lacombe , Martine Bantgratz , Bouma James Neya , Christophe Brugidou , Nicolas Barro (3) , Oumar Traoré (4)

1 INERA - Institut de l'Environnement et Recherches Agricoles [Ouagadougou]
2 UMR IPME - UMR - Interactions Plantes Microorganismes Environnement
3 CRSBAN/UFR-SVT - Laboratoire de Biochimie et Biologie Moléculaire
4 Département de mathématiques

Imperata yellow mottle virus (IYMV, Sobemovirus) was first described in 2008 in the southwestern region of Burkina Faso (West Africa). The genetic diversity of IYMV was not documented up to day. In this study, the variability of CP of IYMV was evaluated through the molecular characterization of 38 isolates collected in the western part of Burkina Faso. Comparison of sequences of these new isolates and one IYMV sequence available in GenBank revealed that the average nucleotide diversity was low. The ratio of non-synonymous over synonymous nucleotide substitutions per site was low, indicating a CP diversification under strong purifying selection. Despite of the low nucleotide diversity, phylogenetic analyses revealed segregation of IYMV isolates into six major clades. There was no correlation of phylogenetic grouping of isolates based on geographical location. This is the first study of the genetic diversity of IYMV.

Mots clés: Imperata yellow mottle virus (IYMV); Phylogenetic Analysis; Genetic Variability; Coat Protein (CP)

Domaines: Phytopathologie et phytopharmacie

Moustapha Koala, Drissa Sérémé, Florence Vignols, Eric Lacombe, Martine Bantgratz, et al.. Molecular Variability and Genetic Structure of IYMV in Burkina Faso. American Journal of Plant Sciences, 2018, 9, pp.311 - 324. ⟨10.4236/ajps.2018.92025⟩. ⟨hal-02414129⟩