Bioassays for detection and identification of cyclic imine toxins

Bio essaies pour la détection et identification d'imines cycliques toxiaues

Romulo Aráoz (1) , Jordi Molgó (2) , Denis Servent (3)

1 INAF - Institut de Neurobiologie Alfred Fessard
2 NBCM - Laboratoire de neurobiologie cellulaire et moléculaire
3 SIMoS - Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO)

Marine neurotoxins move-up fast in the food chain through shellfish that filter-feed on toxic dinoflagellate species constituting a serious hazard for Public Health. Monitoring of regulated marine toxins is performed by physicochemical analyses. It is essential however to develop functional bioassays as an alternative to mouse bioassay for high-throughput detection of novel and emergent marine toxins. Cyclic imine toxins are very-fast acting neurotoxins highly lethal to mice acting as potent antagonists of nicotinic acetylcholine receptors. Although not regulated, chronic exposition to cyclic imine toxins is of concern given their high affinity for neuronal and muscle nicotinic receptors and their capacity to cross the intestinal and the brain blood barriers. A series of bioassays based on the mode of action of cyclic imine toxins are here reviewed. Proceedings of the 18th International Conference on Harmful Algae 21-26 October 2018 Nantes, France.

Mots clés: Cyclic imine toxins; gymnodimines; spirolides; pinnatoxin; receptor-binding assay; toxin fishing

Domaines: Sciences du Vivant [q-bio]; Toxicologie; Sciences de l'environnement; Milieux et Changements globaux

Romulo Aráoz, Jordi Molgó, Denis Servent. Bioassays for detection and identification of cyclic imine toxins. The 18th International Conference on Harmful Algae: From Ecosystems to Socio-Ecosystems., Oct 2018, Nantes, France. ⟨hal-02413151⟩